If placed in a suitable nutrient environment, cells and tissues of many organisms are ableto reproduce and form new plants or animals. Now, we will deal with vegetable tissues,whose culture is simpler than that of animal cellules and tissues. It is necessary toprepare a nutritive and sterilized culture medium for the piece of plant tissue. Keep theculture in the suitable conditions of light and temperature and which vary from plant toplant. Over many days, you will observe the growth of a callus or roots or shoots. In thisway you can obtain even whole plants (cloning). These experiments show that special cellskeep all the information necessary to generate the whole plant.
As we have mentioned, it is necessary avoid bacteria and moulds in the cultures. For thisyou will need sterilize tools, vials, tubes, and nutrient medium. Place each in anautoclave for a ten minutes or, lacking an autoclave, a pressure cooker. The tissues aswell have to be free from microorganisms and they have to be sterilized with bleach (40%solution for 15 min) or with alcohol.
The transfer of the tissues into the test tubes has to be made in aseptic conditions,using a sterile box. Lacking that, make your first trials in a quiet place, as devoid ofwind and dust as possible. The culture medium should contain water, vitamins (particularlythose of the B-complex. For this, use yeast extract), sugars, mineral salts. To enrich thewater with mineral salts, boil some water with a handful of soil, then let settle andfilter it. Usually, people also insert 0.5-0.8% of agar-agar to "solidify" themedium. As culture medium, coconut milk has been used. It contains mineral salts, sugars,vitamins and growth hormones.
1 - For yours first tests of micropropagation, use strawberries tissues.
2 - If this simple experiment interests you, you can continue on the way of the invitro culture of vegetable tissues. In fact you can propagate a lot of plants in thisway. Plants easy to culture are the following: tomato, potato, strawberry, chrysanthemum,geranium, sunflower, tobacco, carrot and onion. You can use tissues obtained from seeds,such as the embryo, but you can use also tissues taken from adult plants, such as tissuesof roots, stems, apical buds, shoots, leaves, even single cells. Each plant and tissue hasits own needs. They are different from each other. You can try the influence of thevegetable hormones, special nutrients, etc.
This field is very broad and complex so, if you are interested in continuing with theseexperiments, you can buy special books and you should build a sterile box.
Plant Tissue Culture for the Gardener
Basic Principle in Plant Tissue Culture Technique
Plant Tissue Culture Kit Manual
Plant Micropropagation Using African Violet Leaves
Plant Tissue Culture (links)
Internet keywords: in vitro culture plant tissue micropropagation.
Making an aquarium or a terrarium is not a complicated thing. Learning to build them isvery useful for those who are fond of breeding animals, observing them, taking picturesand movies. You can use an aquarium to breed fish, amphibians, protists, algae, artemia,shrimps, etc. You can use a terrarium to breed many species of animals, but avoid raisingspecies which suffer in captivity. Aquaria and terrariums can be used also to take picturesof animals temporarily taken from their environment.
In their simpler form, acquaria and terrariums are simple glass boxes enclosed on 5 sidesand which can be provided with a lid. This simple container is suitable to breedamphibians and as a terrarium. Aquaria for fish require a compartment for a filter, a pumpfor water circulation, another pump for air, a thermostat, lighting, etc. We'll deal ofthe simplest aquaria.
How you can build an aquarium? In first you have to make a drawing. The bottom glass hasto be surrounded by the other four walls and it has to be the thickest. The thickness ofthe plates has to be proportioned to the size of the aquarium. Avoid building too wide,and more importantly, too high an aquarium. The pressure of the water increases and couldunglue the plates. After having drawn the acquarium you have to cut the glass plates bymeans of a glasscutter. To do this keep the glasscutter vertical and push down with forcewhile you move it along a ruler. This operation require practice, so use some scrap piecesuntil you have the necessary manual ability. It is important you use a sharp glasscutter,kept in oil to avoid rust. Before gluing the slabs, you have to round off the edges withsandpaper in order to remove their sharp edges. With some acetone, clean the slabs wherethe silicone rubber will be deposited. As the acetone is toxic, work outdoor or in a wellventilate piece. Use high quality transparent silicone. To keep the internal walls free ofexcessive glue, on the slabs and near the gluing positions, place stripes of sticking tape(figure 6). When the silicone is cured, remove these stripes and the excess glue.
: handling and cutting glass is a dangerousoperation which has to be made only by adult people wearing gloves and a heavy apron. Away to elegantly solve this problem is to order plates. A hardware or building supplystore can help. Never move aquaria and terrariums containing water or stones, move them onlywhen they are empty. Do not place aquaria in positions where a water leak could damagesomething below them, for example over electrical devices or sockets, and books. Onlyadults should handle glass-made aquaria or terrariums. Give children transparent plastictanks. Do not keep dangerous or rare organisms. Do not breed and most important, do notfree species which do not belong to your environment! Do not free sick animals or plants.
Reef Aquarium Guide
CyberAqua (list of links)
Internet keywords: aquarium glass homemade.
Demonstrating Oxygen Evolution During Photosynthesis …
In its simpler form, this experiment is particularly suited to elementary schools.
1 - Put some beans in a little jar containing some moist cotton wool. Keep the jar closedto maintain the humidity. Every day pull out the cotton and observe the state of the seedsand measure the length of their roots.
2 - Students in junior high schools or high schools can try to evaluate the influence ofparameters such as temperature, light and nutrients on the speed of germination of theseeds. If seeds are placed in gelatin, it is possible to observe the germination withoutextracting them from the jar.
3 - You can also collect seeds of different plants and determine their vitality (percentof seeds which germinate) as a function of time to harvest.
Exploring Seed Germination
Germinating Seeds on Gelatin
The Great Seed Mystery For Kids
The Wonderful World of Seeds
Internet keywords: germination seeds student.