β-Glycerolphosphate (Glycerol-2-Phosphate) is used in the development of hydrogels and scaffolds that have applications in tissue engineering and cell growth and differentiation. It has also been used as an additive in isolation mediums, similarly providing available phosphate to the isolate. It is a phosphate group donor in matrix mineralization studies that acts as a protein phosphatase inhibitor. Accelerates calcification in vascular smooth muscle cells.
Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 220.127.116.11) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF alleles. In vitro, thermosensitivity of CGPTase strongly suggests that the structural gene for this enzyme is tagF. We discuss apparent discrepancies between biochemical evidence favoring a membrane location for TagF and a previous report that suggested a cytoplasmic location based on sequence analysis.
beta-Glycerophosphate disodium saltProduct Citations
The transfer of electrons is carried out in three steps. In the first step NADH is oxidized by dihydroxyacetone phosphate. In the second step the 3-phosphoglycerol is converted back to dihydroxyacetone by a FAD-depended dehydrogenase located on the inner mitochondrial membrane's outer surface. In the third step the electrons pass from FADH2 to the electron transport chain and the coenzyme is oxidized in the process.