Poster Titles - Discovery On Target

Which of the two reagents should be used depends on the other compounds present in the mixture. Sodium hydroxide is usually easier to handle because it does not evolve carbon dioxide as a byproduct. In addition, the concentration can be increased significantly if is needed. However, if compounds were present that are sensitive towards strong bases or nucleophiles (i.e., esters, ketones, aldehydes, etc.), sodium bicarbonate should be used. It does not react with these compounds because it is a weaker base and a weak nucleophile (due to its resonance stabilization). Note that the formation of carbon dioxide as a byproduct causes a pressure build-up in the separatory funnel, the centrifuge tube or the conical vial. Thus, additional precautions (i.e., frequent venting) have to be taken to prevent any accidents resulting from the pressure build up in the extraction vessel. The target compound can subsequently be recovered by adding a mineral acid to the basic extract i.e., benzoic acid in the Grignard experiment in Chem 30CL.



Target Molecule of The Week - TMTW #1 : chemistry

Use your mouse to right-click (Mac users may need to Ctrl-click) the link above and choose the option that will save the file or target to your computer.


Target Molecule of The Week - TMTW #1 ..

I'll take the liberty of proposing the first week target molecule, ..

AB - OA-519 is a prognostic molecule found in tumor cells from breast cancer patients with markedly worsened prognosis. We purified OA-519 from human breast carcinoma cells, obtained its peptide sequence, and unambiguously identified it as fatty acid synthase through sequence homology and enzymology. Tumor fatty acid synthase is an ≃270-kDa polypeptide which specifically abolished immunostaining of human breast cancers by anti-OA-519 antibodies. Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA- dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. Cerulenin inhibited acylglycerol synthesis in tumor cells and fibroblast controls in a dose-dependent fashion and also caused a growth inhibition which generally paralleled the level of endogenous fatty acid synthesis. Supraphysiologic levels of palmitate, 14 μM in dimethyl sulfoxide, significantly reversed the growth inhibition caused by cerulenin at concentrations of up to 5 μg/ml, indicating that cerulenin- mediated growth inhibition was due to fatty acid synthase inhibition.


10/01/2018 · We’re asking for your help

Our solutions are based on in-depth industrial experience and innovative processes. These help you to meet your Cost of Goods target and generate valuable new IP rights.

Microwave Synthesis - Organic Chemistry Portal

OA-519 is a prognostic molecule found in tumor cells from breast cancer patients with markedly worsened prognosis. We purified OA-519 from human breast carcinoma cells, obtained its peptide sequence, and unambiguously identified it as fatty acid synthase through sequence homology and enzymology. Tumor fatty acid synthase is an ≃270-kDa polypeptide which specifically abolished immunostaining of human breast cancers by anti-OA-519 antibodies. Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA- dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. Cerulenin inhibited acylglycerol synthesis in tumor cells and fibroblast controls in a dose-dependent fashion and also caused a growth inhibition which generally paralleled the level of endogenous fatty acid synthesis. Supraphysiologic levels of palmitate, 14 μM in dimethyl sulfoxide, significantly reversed the growth inhibition caused by cerulenin at concentrations of up to 5 μg/ml, indicating that cerulenin- mediated growth inhibition was due to fatty acid synthase inhibition.

Synthesis of Radiolabeled Compounds

Retrosynthesis or retrosynthetic analysis is a strategy for planning an organic synthesis by disconnecting a target molecule into precursor materials. This steps are repeated until available starting materials are reached.