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Effect of ANK-199 on autophagic deathin CAR cells. Cells in the presence and absence of ANK-199challenge for 24 h were harvested. (A) The autophagolysosome markerwas probed by MDC. (B) AO staining was used to detect AVO. (C) LC3Bexpression was determined by Premo Autophagy Sensor kit. (D) DNAcondensation (an apoptotic characteristic) was tested utilizingDAPI staining.
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