Sampling and Analytical Methods: Benzyl Acetate, …

(1954) Rat Intravenous (Na salt) 1714 ± 124 Spector (1956) Rat Oral (acid) 2000-2500 Ignat'ev (1965) Rabbit Oral (Na salt) 2000 Spector (1956) Rabbit Subcutaneous (Na salt) 2000 Spector (1956) Dog Oral (Na salt) 2000 Spector (1956) Benzyl acetate Groups of five B6C3F1 mice or Fischer 344 rats of each sex were administered single doses of benzyl acetate (purity, 96.0-101.3%) at doses of 250, 500, 1000, 2000, or 4000 mg/kg bw in corn oil by gavage and were observed for 15 days.

The authors in terpreted their result as indicating a weak promoting activity of benzyl acetate.

2.2.6 Genotoxicity The results of studies of the genotoxicity of benzyl acetate, benzyl alcohol, benzaldehyde, benzoic acid and benzoate salts, the metabolite, and hippuric acid are presented in Table 3.


Sampling and Analytical Methods, Benzyl Acetate, PV2124

The authors concluded that benzyl acetate is not teratogenic and suggested that the increased incidences of skeletal and internal variations were due to slight maternal toxicity and to the significant decrease in fetal body weight.


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Feed was prepared weekly, stored in the dark, and analysed during the study for benzyl acetate concentration, stability, and homogeneity; it contained low, biologically insignificant levels of aflatoxins, pesticides, and heavy metals.

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Groups of 60 male and 60 female Fischer 344 rats, of an average of 41 days at initial exposure (12 days' quarantine before the test), received benzyl acetate (properties consistent with structure and literature references; purity, >98%; stability monitored periodically, and no degradation of bulk chemical observed) at doses of 0, 3000, 6000, or 12 000 mg/kg feed, equal to 0, 130, 260, and 510 mg/kg bw per day for males and 0, 145, 290, and 575 mg/kg bw per day for females, for 103 weeks.

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Groups of 60 male and 60 female B6C3F1 mice, with an average age at initial exposure of 40 days (11 days of quarantine before test), received diets containing benzyl acetate (properties consistent with structure and literature references; purity, > 98%; stability monitored periodically, and no degradation of bulk chemical observed) at a dose of 0, 330, 1000, or 3000 mg/kg feed, equal to 0, 37, 112, and 346 mg/kg bw per day for males and 0, 42, 132, and 382 mg/kg bw per day for females, for 103 weeks.

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2.2.3 Long-term toxicity and carcinogenicity 2.2.3.1 Mice Benzyl acetate Groups of 50 B6C3F1 mice of each sex, aged eight weeks, were given benzyl acetate (purity, 99-101%) in corn oil by gavage at a dose of 500 or 1000 mg/kg bw per day on five days per week for 103 weeks.

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(con't) End-point Test object Concentration Result Reference Benzyl acetate (con't) Sister chromatid Chinese hamster 50-5000 µg/ml Negativeb US National exchange ovary cells Toxicology Program (1986, 1993); Galloway et al.