Synthesis of silver nanoparticles:

Part A: Molecular and Biomolecular Spectroscopy; citation_title=Green synthesis of silver nanoparticles using Macrotyloma uniflorum.; citation_author=V.

GREEN SYNTHESIS OF NANOPARTICLES AND THEIR ANTIBACTERIAL ACTIVITY AGAINST PATHOGENIC BACTERIA

Part A: Molecular and Biomolecular Spectroscopy; citation_title=Green synthesis of SnO2 nanoparticles and its photocatalytic activity of phenolsulfonphthalein dye.; citation_author=G.


Preparation of plant broth and biosynthesis of silver nanoparticles

Figure 2: UV-Vis spectra of silver nanoparticles prepared at different temperatures.

The protocol for the nanoparticle syntheses involves: the collection of the part of plant of interest from the available sites then it’s washing thoroughly with tap water to remove contamination followed by surface sterilization with double distilled water and air dried at room temperature. These clean and fresh sources are then powdered using domestic blender or cut it into very small pieces. And for the plant broth preparation, around 10-25g of the dried powder or finally chopped leaves were kept in a beaker and boiled with 100mL of deionised distilled water. The extract was filtered with Whatman filter paper No.1 further the filtrate was used as reducing source for the synthesis of silver nanoparticles.


exploited in the synthesis of silver nanoparticles ..

Silver nanoparticles (AgNPs) were prepared in a one-step microwave-assisted synthesis guided by the principles of green chemistry. Microwave parameters were optimized using the Box–Benhken design for three factors (time, temperature, and pressure). Aqueous extracts from the peels of citrus fruits (orange, grapefruit, tangelo, lemon, and lime) were used for the synthesis of AgNPs using microwave technology, though the synthesis of AgNPs was only successful using the orange peel extract. Nanospheres of TEM mean diameter (with standard deviation) of 7.36 ± 8.06 nm were successfully synthesized in 15 min by reducing Ag+ ions (from AgNO3) with orange peel extract, which also served as a capping agent. Creation of AgNPs was confirmed using UV–visible spectroscopy, fluorescence emission spectroscopy, powder X-ray diffraction, and transmission electron microscopy, while size analysis was gathered from both transmission electron microscopy as well as dynamic light scattering. Analysis of all citrus peel extracts by gas chromatography–mass spectrometry indicated that the putative compounds responsible for successful AgNP synthesis with orange extract were aldehydes. The creation of AgNPs using environmentally benign reagents in minimal time paves the way for future studies on AgNP toxicity without risking interference from potentially toxic reagents and capping agents.

synthesis of silver nanoparticles by using plants.

Metal nanoparticles have received a lot of attention in the recent decades due to their unique ability to alter optical, electrical, biological properties [1-5]. Nanoparticles are also interesting because of their huge increase in surface area and a surge in conductivity compared to their metal counterparts. Moreover, metal nanoparticles also show surface plasmon resonance. These properties make them useful to many applications such as bio-sensing, imaging, drug delivery, HIV treatment, Cancer treatment, optical spectroscopy and Surface Enhanced Raman scattering (SERS) [2-4].

Green synthesis of nanoparticles - SlideShare

One of the potential application of Ag NPs is the determination of hydrogen peroxide. Hydrogen peroxide has great relevance pertaining to environmental, pharmaceutical and clinical research [22-24]. Hydrogen peroxide is a very good oxidizing agent. In addition, hydrogen peroxide is a byproduct of number of enzymatic reaction involving glucose, cholesterol and lactate. However, higher concentration and amount of hydrogen peroxide can induce potentially serious disease in human body and can cause diseases such as cancer etc. The high concentration rates of hydrogen peroxide can lead to these problems. Thus a stable method for the sensing of different concentrations of hydrogen peroxide has to be developed. Many methods for the sensitive determination of hydrogen peroxide has been developed such as chemiluminescence, optical detection and electrochemical method. Of these, optical detection is found to be the best method as it is convenient and low cost. In the present manuscript, we synthesized silver nanoparticle by green route. The silver nanoparticle synthesized were characterized and coated on a cellulose substrate for optical detection of hydrogen peroxide.