Cuvette 5 also showed no change in the percentage of light transmittance because without the presence of chloroplast, the light could not be absorbed to excite the elections and to reduce the DPIP. Without the functions of chloroplast, photosynthesis could not occur because the DPIP would not be reduced and ATP would not be created. Any fluctuations in the data or graph for cuvette 5 could be explained by human or data error.
Without functional chloroplast to absorb the energy from the light, the electrons will not be bumped to a higher energy level and will not be able to reduce DPIP. Of the two cuvettes with unboiled chloroplast, the cuvette place in front of the light will have a higher percent of light transmittance than the cuvette placed in the dark because with light, energy can be absorbed, DPIP can be reduced, ATP can be created, and photosynthesis can be carried out.
18/12/2017 · Dexter Luu's AP labs
The purpose of this lab is to measure the effect of various conditions of chloroplast on the rate of photosynthesis or percentage of light transmittance. By using unboiled chloroplast in light, unboiled chloroplast in dark, and boiled chloroplast in light, DPIP was placed into each cuvette and a colorimeter was used to measure the rate of light transmittance.