The ADSCs growing on the microcarriers and those inmonolayer culture were harvested, digested and smeared. They werethen subjected to safranin-O and toluidine blue staining to observethe levels of glycosaminoglycan (GAG) synthesis and secretion fromthe extracellular matrix. The type II collagen monoclonal antibodywas incubated with the cells with DAB (Beijing Hepten and ProteinBiomedical Institute, Beijing, China) as the substrate, and theimmunoperoxidase method was used for development. Brown stainingindicated positive staining, and thus the occurrence of type IIcollagen secretion and synthesis.
In conclusion, this type of dynamic culturemicroenvironment provides cell aggregation, fast three-dimensionalgrowth and effective differentiation conditions, and provides themost effective method for tissue engineering. The results of thecurrent study confirmed this. In the present study, the ADSCsquickly attached, extended and rapidly proliferated on themicrocarriers. On day 7, cells covered the surfaces of themicrocarriers completely, and grew, projecting out from themicrocarriers. The microcarriers connected with each other andformed masses. The number of cells in the microcarrier cultureincreased by almost 20-fold in one week, while that of the staticculture increased only by 6-fold, and histochemical andimmunohistochemical analysis indicated that microcarrier culturecells possessed improved phenotypes and extracellular matrixproduction. The current study used ADSCs as seed cells to constructcartilage tissue, providing an experimental basis, and thefoundation for future investigation of cartilage tissueengineering.
Type I collagen synthesis by corneal endohelial cells modulated by ..
Collagen is a major component of the extracellular matrix (ECM). As such, it is a natural choice for biological coatings in many applications. Type I collagens are most frequently used for coatings. Stents can exude drugs and genes. To fix drugs to the stent, medical device manufacturers combine them with collagen and spray the mixture in thin layers on the wall of the device. Because collagen is naturally absorbed, it can be manufactured for controlled release of the embedded drug or nucleotides.
04.01.2018 · Anti-Collagen I antibody (ab34710) ..
BioCell Collagen was well tolerated and found to be effective in managing OA-associated symptoms over the study period, thereby improving patient's activities of daily living.
Proline Precursors to Sustain Mammalian Collagen Synthesis
Each UC-II (InterHealth Nutraceuticals, Inc., Benicia, CA) capsule contained 20 mg UC-II standardized to 5 mg of bioactive undenatured type II collagen. Subjects in the UC-II group were instructed to take two “sugar pills” in the morning to protect blinding and two UC-II capsules in the evening accounting for a daily dose of 40 mg UC-II containing 10 mg of bioactive undenatured type II collagen.
Collagen Types and Linked Disorders - News Medical
The nutritional supplement, Pure Gold Collagen(®), which consists of hydrolyzed collagen, hyaluronic acid, vitamins, and minerals, was developed to counteract these signs.
15.07.2013 · Collagen Types and Linked Disorders
On days 1, 3, 5 and 7, ADSCs were harvested from theRCCS container and common culture bottle and were counted, and thegrowth curves were as presented in . The Trypan blue dye exclusion testindicated that the survival rate of ADSCs harvested from themicrocarriers was >90%. According to the blood cell count, itwas clear that the growth of ADSCs accelerated from day 3 afterRCCS inoculation, and the cell density reached its highest value atday 7, ~19 times the initial inoculation. On day 7, the totalnumber of harvested cells cultured in a static monolayer was ~6times the original.
US5500409A - Method for inhibiting collagen synthesis …
The Cytodex 3 microcarriers (50 mg) and ADSCs (finalconcentration, 1×10/ml) were added to a 10-ml rotarycell culture system (RCCS; Synthecon, Inc., Houston, TX, USA)culture container, then the cartilage inducing medium was added tofill the container. The container with cell-microcarriers complexwas connected to the rotating base. The device was put in a 5%CO incubator and was cultured at 37°C. In order tofully mix cells, the container was rotated at a force of 5 rpm for5 min and paused for 5 min alternately, which was repeated forseveral cycles, it was then continuously rotated and graduallyadjusted to 10–12 rpm. Once the culture medium exhibited a yellowcolor and a PH value /ml ADSCs intofive wells of a 6-well plate, 2 ml in each well. During theculture, the medium was changed every 2–3 days to maintain asufficient quantity of cells.