N2 - A simple and convenient synthesis of cholesterol esters from cholesterol and fatty acid anhydrides is described. The high, reproducible yield of cholesterol ester and the small excess of fatty acid used makes this method attractive for the preparation of cholesterol esters using difficulty obtained fatty acids.
Cholesterol esterase is synthesized in the acinar cells of the pancreas, stored in zymogen granules, and secreted as a component of pancreatic juice into the lumen of the small intestine (Labow et al. 1983). Cholesterol esterase hydrolyzes a wide range of ester substrates including cholesteryl esters, acylglycerides, phospholipids (Brockerhoff and Jensen 1974), retinyl esters (Fredrikzon et al. 1978), vitamin esters, and phenyl esters (Rudd and Brockman 1984). The enzyme has also been found to have amidase activity (Hui et al. 1993). The enzyme is useful as a biocatalyst because of its ability to catalyze transacylation reactions in a water-limited environment (Gallo et al. 1977, Kazlauskas 1989, and Feaster et al. 1996).
in fat and carbohydrate and thereby increasing cholesterol synthesis.
Throughout the early 1900s the enzymatically catalyzed synthesis and hydrolysis of cholesterol esters in the presence of certain tissues was observed and described (Kondo 1910, Schultz 1912, Cytronberg 1912, Gardner and Lander 1913, Mueller 1916, Porter 1916, Nomura 1924, Shope 1928, Nedswedski 1935, Klein 1939, Sperry 1935, and Sperry and Stoyanoff 1937).